产品描述

This Human LEFTY2 ELISA Kit was designed for the quantitative measurement of Human LEFTY2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

别名

EBAF ELISA Kit; Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily) ELISA Kit; Endometrial bleeding associated factor ELISA Kit; Endometrial bleeding-associated factor ELISA Kit; Left right determination factor 2 ELISA Kit; Left right determination factor A ELISA Kit; Left-right determination factor 2 ELISA Kit; Left-right determination factor A ELISA Kit; LEFTA ELISA Kit; LEFTY 2 ELISA Kit; LEFTY2 ELISA Kit; LEFTYA ELISA Kit; LFTY2 transforming growth factor; beta -4 ELISA Kit; LFTY2; mouse; homolog of ELISA Kit; LFTY2_HUMAN ELISA Kit; MGC46222 ELISA Kit; Protein lefty 2 ELISA Kit; Protein lefty A ELISA Kit; Protein lefty-2 ELISA Kit; Protein lefty-A ELISA Kit; Protein lefty2 ELISA Kit; PSEC0024 ELISA Kit; TGF beta 4 ELISA Kit; TGF-beta-4 ELISA Kit; TGFB4 ELISA Kit; Transforming growth factor beta 4 ELISA Kit; Transforming growth factor beta-4 ELISA Kit

缩写

LEFTY2

Uniprot No

O00292

灵敏度

1.95 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LEFTY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		91
		Range %		86-95
1:200		Average %		102
		Range %		97-107
1:400		Average %		91
		Range %		85-97
1:800		Average %		97
		Range %		91-103

回收率

The recovery of human LEFTY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 500 2.811 2.836 2.824 2.744 250 2.038 2.000 2.019 1.939 125 1.331 1.368 1.350 1.270 62.5 0.804 0.752 0.778 0.698 31.2 0.473 0.498 0.486 0.406 15.6 0.278 0.281 0.280 0.200 7.8 0.173 0.169 0.171 0.091 0 0.079 0.081 0.080

货期

3-5 working days