产品描述

This Human LTA ELISA Kit was designed for the quantitative measurement of Human LTA protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

LTalpha ELISA Kit; DAMA-25N12.13-004 ELISA Kit; DIF ELISA Kit; LT alpha ELISA Kit; LT ELISA Kit; LT-alpha ELISA Kit; Lta ELISA Kit; Lymphotoxin alpha (TNF superfamily, member 1) ELISA Kit; Lymphotoxin alpha ELISA Kit; Lymphotoxin-alpha ELISA Kit; MGC117668 ELISA Kit; OTTHUMP00000037612 ELISA Kit; OTTHUMP00000037613 ELISA Kit; TNF B ELISA Kit; TNF superfamily member 1 ELISA Kit; TNF, lymphocyte-derived ELISA Kit; TNF-beta ELISA Kit; TNFB ELISA Kit; TNFB_HUMAN ELISA Kit; TNFbeta ELISA Kit; TNFSF 1 ELISA Kit; TNFSF1 ELISA Kit; TNLG1E ELISA Kit; Tumor necrosis factor beta ELISA Kit; tumor necrosis factor ligand 1E ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit; tumor necrosis factor superfamily member 1 ELISA Kit

缩写

LTA

Uniprot No

P01374

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		83-99
1:2		Average %		87
		Range %		84-93
1:4		Average %		96
		Range %		90-104
1:8		Average %		93
		Range %		86-102

回收率

The recovery of human TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-100
EDTA plasma (n=4)		97		90-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 1.823 1.911 1.867 1.779 500 1.315 1.376 1.346 1.258 250 0.807 0.816 0.812 0.724 125 0.533 0.573 0.553 0.465 62.5 0.324 0.308 0.316 0.228 31.2 0.259 0.246 0.253 0.165 15.6 0.159 0.163 0.161 0.073 0 0.086 0.090 0.088

货期

3-5 working days