产品描述
This Rabbit MMP3 ELISA Kit was designed for the quantitative measurement of Rabbit MMP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
MMP3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit
缩写
MMP3
Uniprot No
P28863
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
84 |
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| Range % |
80-94 |
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| 1:10 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:20 |
Average % |
93 |
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| Range % |
85-97 |
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| 1:40 |
Average % |
94 |
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| Range % |
86-99 |
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回收率
| The recovery of rabbit MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
89-98 |
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| EDTA plasma (n=4) |
89 |
85-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.794 |
2.694 |
2.744 |
2.593 |
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| 5 |
2.244 |
2.144 |
2.194 |
2.043 |
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| 2.5 |
1.424 |
1.324 |
1.374 |
1.223 |
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| 1.25 |
0.854 |
0.824 |
0.839 |
0.688 |
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| 0.625 |
0.546 |
0.536 |
0.541 |
0.390 |
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| 0.312 |
0.361 |
0.351 |
0.356 |
0.205 |
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| 0.156 |
0.230 |
0.220 |
0.225 |
0.074 |
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| 0 |
0.152 |
0.149 |
0.151 |
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货期
3-5 working days