产品描述

This Human NBL1 ELISA Kit was designed for the quantitative measurement of Human NBL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 34.4 pg/mL-2200 pg/mL and the sensitivity is 8.6 pg/mL.

别名

D1S1733E ELISA Kit; DAN ELISA Kit; DAN domain family member 1 ELISA Kit; DAND 1 ELISA Kit; DAND1 ELISA Kit; Differential screening selected gene aberrant in neuroblastoma ELISA Kit; MGC8972 ELISA Kit; NB ELISA Kit; NBL 1 ELISA Kit; NBL1 ELISA Kit; NBL1_HUMAN ELISA Kit; Neuroblastoma candidate region suppression of tumorigenicity 1 ELISA Kit; Neuroblastoma suppression of tumorigenicity 1 ELISA Kit; Neuroblastoma suppressor of tumorigenicity 1 ELISA Kit; NO 3 ELISA Kit; NO3 ELISA Kit; Protein N03 ELISA Kit; Zinc finger protein DAN ELISA Kit

缩写

NBL1

Uniprot No

P41271

灵敏度

8.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NBL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		84-96
1:2		Average %		97
		Range %		91-105
1:4		Average %		103
		Range %		97-107
1:8		Average %		89
		Range %		83-94

回收率

The recovery of human NBL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		94-105
EDTA plasma (n=4)		106		101-110

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 2200 1.755 1.821 1.788 1.665 1100 1.285 1.289 1.287 1.164 550 0.848 0.845 0.847 0.724 275 0.547 0.533 0.540 0.417 137.5 0.353 0.365 0.359 0.236 68.75 0.298 0.321 0.310 0.187 34.4 0.225 0.214 0.220 0.097 0 0.125 0.121 0.123

货期

3-5 working days