产品描述
This Pig IL10 ELISA Kit was designed for the quantitative measurement of Pig IL10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .
别名
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写
IL10
Uniprot No
Q29055
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 81-103 | |
| 1:2 | Average % | 95 | |
| Range % | 90-105 | |
| 1:4 | Average % | 95 | |
| Range % | 90-108 | |
| 1:8 | Average % | 96 | |
| Range % | 82-112 | |
回收率
| The recovery of pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 85-109 | |
| EDTA plasma (n=4) | 97 | 92-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.178 | 2.152 | 2.165 | 2.015 | | | 200 | 1.584 | 1.560 | 1.572 | 1.422 | | | 100 | 0.981 | 0.950 | 0.966 | 0.816 | | | 50 | 0.568 | 0.564 | 0.566 | 0.416 | | | 25 | 0.372 | 0.361 | 0.367 | 0.217 | | | 12.5 | 0.296 | 0.302 | 0.299 | 0.149 | | | 6.25 | 0.252 | 0.248 | 0.250 | 0.100 | | | 0 | 0.152 | 0.148 | 0.150 | | |
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货期
3-5 working days