产品描述
This Rat OLR1 ELISA Kit was designed for the quantitative measurement of Rat OLR1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
Olr1; Lox1; Oldlr1; Oxidized low-density lipoprotein receptor 1; Ox-LDL receptor 1; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1
缩写
OLR1
Uniprot No
O70156
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sLOX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 85-94 | |
| 1:2 | Average % | 92 | |
| Range % | 88-99 | |
| 1:4 | Average % | 88 | |
| Range % | 84-92 | |
| 1:8 | Average % | 95 | |
| Range % | 90-100 | |
回收率
| The recovery of rat sLOX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 85 | 80-90 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.375 | 2.227 | 2.301 | 2.190 | | | 5 | 1.816 | 1.902 | 1.859 | 1.748 | | | 2.5 | 1.240 | 1.208 | 1.224 | 1.113 | | | 1.25 | 0.725 | 0.785 | 0.755 | 0.644 | | | 0.625 | 0.421 | 0.439 | 0.430 | 0.319 | | | 0.312 | 0.263 | 0.284 | 0.274 | 0.163 | | | 0.156 | 0.218 | 0.221 | 0.220 | 0.109 | | | 0 | 0.109 | 0.112 | 0.111 | | |
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货期
3-5 working days