产品描述
This Human MT-ND6 ELISA Kit was designed for the quantitative measurement of Human MT-ND6 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.
别名
MT-ND6 ELISA Kit; MTND6 ELISA Kit; NADH6 ELISA Kit; ND6 ELISA Kit; NADH-ubiquinone oxidoreductase chain 6 ELISA Kit; EC 7.1.1.2 ELISA Kit; NADH dehydrogenase subunit 6 ELISA Kit
缩写
MT-ND6
Uniprot No
P03923
灵敏度
5.86 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of Human MT-ND6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 84-98 | |
| 1:2 | Average % | 86 | |
| Range % | 80-92 | |
| 1:4 | Average % | 94 | |
| Range % | 85-98 | |
| 1:8 | Average % | 102 | |
| Range % | 98-106 | |
回收率
| The recovery of Human MT-ND6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 88-98 | |
| EDTA plasma (n=4) | 89 | 83-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 2.763 | 2.867 | 2.815 | 2.716 | | | 750 | 2.426 | 2.321 | 2.374 | 2.275 | | | 375 | 1.675 | 1.772 | 1.724 | 1.625 | | | 187.5 | 1.045 | 1.115 | 1.080 | 0.981 | | | 93.75 | 0.527 | 0.534 | 0.531 | 0.432 | | | 46.88 | 0.274 | 0.277 | 0.276 | 0.177 | | | 23.44 | 0.188 | 0.178 | 0.183 | 0.084 | | | 0 | 0.098 | 0.099 | 0.099 | | |
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货期
3-5 working days