产品描述
This Rabbit MMP1 ELISA Kit was designed for the quantitative measurement of Rabbit MMP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
MMP1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit
缩写
MMP1
Uniprot No
P13943
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-100 | |
| 1:2 | Average % | 104 | |
| Range % | 97-109 | |
| 1:4 | Average % | 88 | |
| Range % | 82-92 | |
| 1:8 | Average % | 98 | |
| Range % | 91-102 | |
回收率
| The recovery of rabbit MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 88 | 82-92 | |
| EDTA plasma (n=4) | 102 | 92-107 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.590 | 2.545 | 2.568 | 2.466 | | | 5 | 1.963 | 1.922 | 1.943 | 1.841 | | | 2.5 | 1.435 | 1.400 | 1.418 | 1.316 | | | 1.25 | 0.841 | 0.818 | 0.830 | 0.728 | | | 0.625 | 0.448 | 0.427 | 0.438 | 0.336 | | | 0.312 | 0.328 | 0.317 | 0.323 | 0.221 | | | 0.156 | 0.248 | 0.242 | 0.245 | 0.143 | | | 0 | 0.101 | 0.103 | 0.102 | | |
|
货期
3-5 working days