产品描述
This Human MT-RNR2 ELISA Kit was designed for the quantitative measurement of Human MT-RNR2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.
别名
MT-RNR2 ELISA Kit; HN ELISA Kit; Humanin ELISA Kit; Humanin mitochondrial ELISA Kit; HNM ELISA Kit
缩写
MT-RNR2
Uniprot No
Q8IVG9
灵敏度
7 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MT-RNR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 100 | |
| Range % | 92-105 | |
| 1:10 | Average % | 97 | |
| Range % | 91-105 | |
| 1:20 | Average % | 97 | |
| Range % | 92-106 | |
| 1:40 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of human MT-RNR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 85-99 | |
| EDTA plasma (n=4) | 95 | 90-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1800 | 2.120 | 2.163 | 2.142 | 2.007 | | | 900 | 1.675 | 1.605 | 1.640 | 1.505 | | | 450 | 1.158 | 1.168 | 1.163 | 1.028 | | | 225 | 0.741 | 0.780 | 0.761 | 0.626 | | | 112.5 | 0.352 | 0.383 | 0.368 | 0.233 | | | 56.25 | 0.231 | 0.241 | 0.236 | 0.101 | | | 28 | 0.178 | 0.186 | 0.182 | 0.047 | | | 0 | 0.132 | 0.138 | 0.135 | | |
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货期
3-5 working days