产品描述
This Goat IL12B ELISA Kit was designed for the quantitative measurement of Goat IL12B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.38 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL.
别名
IL12B ELISA Kit; Interleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
缩写
IL12B
Uniprot No
P68221
灵敏度
2.34 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 89-102 | |
| 1:2 | Average % | 86 | |
| Range % | 82-97 | |
| 1:4 | Average % | 93 | |
| Range % | 85-102 | |
| 1:8 | Average % | 95 | |
| Range % | 89-105 | |
回收率
| The recovery of goat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 85 | 81-90 | |
| EDTA plasma (n=4) | 92 | 87-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 600 | 1.974 | 1.942 | 1.958 | 1.857 | | | 300 | 1.331 | 1.362 | 1.347 | 1.246 | | | 150 | 0.912 | 0.924 | 0.918 | 0.817 | | | 75 | 0.587 | 0.612 | 0.600 | 0.499 | | | 37.5 | 0.401 | 0.388 | 0.395 | 0.294 | | | 18.75 | 0.295 | 0.309 | 0.302 | 0.201 | | | 9.37 | 0.214 | 0.225 | 0.220 | 0.119 | | | 0 | 0.102 | 0.100 | 0.101 | | |
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货期
3-5 working days