产品描述
This Human MAP2 ELISA Kit was designed for the quantitative measurement of Human MAP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
别名
DKFZp686I2148 ELISA Kit; MAP 2 ELISA Kit; MAP dendrite specific ELISA Kit; MAP-2 ELISA Kit; MAP2 ELISA Kit; MAP2A ELISA Kit; MAP2B ELISA Kit; MAP2C ELISA Kit; Microtubule associated protein 2 ELISA Kit; Microtubule-associated protein 2 ELISA Kit; MTAP2_HUMAN ELISA Kit
缩写
MAP2
Uniprot No
P11137
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 86-95 | |
| 1:2 | Average % | 104 | |
| Range % | 100-107 | |
| 1:4 | Average % | 92 | |
| Range % | 87-95 | |
| 1:8 | Average % | 97 | |
| Range % | 91-102 | |
回收率
| The recovery of human MAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-96 | |
| EDTA plasma (n=4) | 97 | 90-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 1.626 | 1.613 | 1.620 | 1.528 | | | 5 | 1.194 | 1.176 | 1.185 | 1.093 | | | 2.5 | 0.848 | 0.832 | 0.840 | 0.748 | | | 1.25 | 0.602 | 0.598 | 0.600 | 0.508 | | | 0.625 | 0.428 | 0.412 | 0.420 | 0.328 | | | 0.312 | 0.264 | 0.286 | 0.275 | 0.183 | | | 0.156 | 0.158 | 0.154 | 0.156 | 0.064 | | | 0 | 0.093 | 0.091 | 0.092 | | |
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货期
3-5 working days