产品描述
This Mouse IL31 ELISA Kit was designed for the quantitative measurement of Mouse IL31 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .
别名
Il31Interleukin-31 ELISA Kit; IL-31 ELISA Kit
缩写
IL31
Uniprot No
Q6EAL8
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 82-99 | |
| 1:2 | Average % | 97 | |
| Range % | 87-105 | |
| 1:4 | Average % | 95 | |
| Range % | 88-100 | |
| 1:8 | Average % | 90 | |
| Range % | 82-98 | |
回收率
| The recovery of mouse IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 93-104 | |
| EDTA plasma (n=4) | 99 | 90-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.886 | 2.846 | 2.866 | 2.702 | | | 1000 | 2.347 | 2.336 | 2.342 | 2.178 | | | 500 | 1.559 | 1.602 | 1.581 | 1.417 | | | 250 | 0.897 | 0.878 | 0.888 | 0.724 | | | 125 | 0.532 | 0.557 | 0.545 | 0.381 | | | 62.5 | 0.366 | 0.374 | 0.370 | 0.206 | | | 31.25 | 0.262 | 0.245 | 0.254 | 0.090 | | | 0 | 0.162 | 0.165 | 0.164 | | |
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货期
3-5 working days