产品描述

This human IL-21 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human IL-21 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL-21 levels in the sample and is measured at 450 nm through a microplate reader.

IL-21 plays multiple biologic roles, including maintenance and function of CD8⁺ memory T cells and natural killer cells, as well as promoting the generation of Th17 cells in the mouse. IL-21 also promotes B-cell activation, differentiation, or death during humoral immune responses. IL-21 signals through IL-21R, activating the JAK1/3-STAT signaling pathway that initiates a gene transcription profile such as Gzma, Gzmb, Il10, Eomes, and Rorgt. Furthermore, elevated IL-21 generation is characteristic of certain autoimmune diseases and is likely to contribute to autoantibody production as well as pathologic features of autoimmune disease. In contrast, IL-21 may function as a co-adjuvant to enhance antibody responses and thereby facilitate host defense to malignancies and infectious diseases.

别名

缩写

Uniprot No

灵敏度

反应时间

样本体积

检测波长

研究领域

测定原理

精密度

线性度

回收率

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 200 2.445 2.345 2.395 2.250 100 1.864 1.764 1.814 1.669 50 1.162 1.151 1.157 1.012 25 0.723 0.713 0.718 0.573 12.5 0.432 0.422 0.427 0.282 6.25 0.315 0.305 0.310 0.165 3.12 0.222 0.212 0.217 0.072 0 0.145 0.144 0.145

货期