产品描述
This Mouse IGFBP2 ELISA Kit was designed for the quantitative measurement of Mouse IGFBP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.
别名
Igfbp2 ELISA Kit; Igfbp-2Insulin-like growth factor-binding protein 2 ELISA Kit; IBP-2 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP-2 ELISA Kit; mIGFBP-2 ELISA Kit
缩写
IGFBP2
Uniprot No
P47877
灵敏度
0.11 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:20 |
Average % |
103 |
|
|
| Range % |
98-107 |
|
|
| 1:40 |
Average % |
84 |
|
|
| Range % |
80-88 |
|
|
| 1:80 |
Average % |
96 |
|
|
| Range % |
92-100 |
|
|
| 1:160 |
Average % |
90 |
|
|
| Range % |
86-94 |
|
|
回收率
| The recovery of mouse IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
88 |
84-92 |
|
|
| EDTA plasma (n=4) |
95 |
90-100 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 30 |
2.641 |
2.496 |
2.569 |
2.470 |
|
|
| 15 |
1.750 |
1.625 |
1.688 |
1.589 |
|
|
| 7.5 |
1.004 |
1.080 |
1.042 |
0.943 |
|
|
| 3.75 |
0.638 |
0.659 |
0.649 |
0.550 |
|
|
| 1.8 |
0.329 |
0.313 |
0.321 |
0.222 |
|
|
| 0.9 |
0.215 |
0.204 |
0.210 |
0.111 |
|
|
| 0.45 |
0.162 |
0.171 |
0.167 |
0.068 |
|
|
| 0 |
0.096 |
0.102 |
0.099 |
|
|
|
|
货期
3-5 working days