产品描述
The Human Mimecan (OGN) ELISA kit is a highly sensitive and reliable tool for quantitative analysis of Mimecan protein levels in human serum, plasma, and tissue homogenates. The kit is designed to measure Mimecan, also known as osteoglycin (OGN), a small leucine-rich proteoglycan involved in various cellular processes, including cell adhesion, differentiation,proliferation and bone formation.
The Human Mimecan (OGN) ELISA kit has a broad detection range of 0.156 ng/mL-10 ng/mL, with a sensitivity of 0.039 ng/mL, ensuring accurate and precise measurements even at low concentrations. The assay principle used is sandwich, which involves the use of two monoclonal antibodies specific to the Mimecan protein.
The assay time for the kit is 1-5 hours, and the sample volume required is 50-100ul. Detection is done at a wavelength of 450 nm, making it easy to read and interpret the results. The kit is suitable for use in cell biology and bone formation research and has been cited in over 2 research articles.
别名
Corneal keratan sulfate proteoglycan ELISA Kit; DKFZP586P2421 ELISA Kit; MIME_HUMAN ELISA Kit; Mimecan ELISA Kit; Mimecan proteoglycan ELISA Kit; OG ELISA Kit; OGN ELISA Kit; OIF ELISA Kit; Osteoglycin ELISA Kit; Osteoinductive factor ELISA Kit; SLRR3A ELISA Kit
缩写
OGN
Uniprot No
P20774
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human OGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 96 | |
| Range % | 91-105 | |
| 1:10 | Average % | 95 | |
| Range % | 92-98 | |
| 1:20 | Average % | 87 | |
| Range % | 80-92 | |
| 1:40 | Average % | 99 | |
| Range % | 96-108 | |
回收率
| The recovery of human OGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 89-104 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.432 | 2.332 | 2.382 | 2.254 | | | 5 | 1.804 | 1.704 | 1.754 | 1.626 | | | 2.5 | 1.187 | 1.287 | 1.237 | 1.109 | | | 1.25 | 0.716 | 0.726 | 0.721 | 0.593 | | | 0.625 | 0.447 | 0.457 | 0.452 | 0.324 | | | 0.312 | 0.345 | 0.355 | 0.350 | 0.222 | | | 0.156 | 0.227 | 0.237 | 0.232 | 0.104 | | | 0 | 0.129 | 0.126 | 0.128 | | |
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货期
3-5 working days
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