产品描述
This Mouse MRCC-I ELISA Kit was designed for the quantitative measurement of Mouse MRCC-I protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
缩写
MRCC-I
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MRCC-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 96 | | |
| Range % | 84-100 | | |
| 1:2 | Average % | 100 | | |
| Range % | 94-103 | | |
| 1:4 | Average % | 94 | | |
| Range % | 84-98 | | |
| 1:8 | Average % | 100 | | |
| Range % | 93-103 | | |
回收率
| The recovery of mouse MRCC-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 84-97 | | |
| EDTA plasma (n=4) | 99 | 91-103 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 500 | 2.187 | 2.099 | 2.143 | 1.972 | | | | 250 | 1.549 | 1.476 | 1.513 | 1.342 | | | | 125 | 1.098 | 1.044 | 1.071 | 0.900 | | | | 62.5 | 0.812 | 0.789 | 0.801 | 0.630 | | | | 31.2 | 0.575 | 0.545 | 0.560 | 0.389 | | | | 15.6 | 0.416 | 0.423 | 0.420 | 0.249 | | | | 7.8 | 0.293 | 0.281 | 0.287 | 0.116 | | | | 0 | 0.169 | 0.173 | 0.171 | | | |
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货期
3-5 working days