产品描述

The rat IL-12 ELISA kit is suitable for the quantitative determination of rat IL-12 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

IL-12 is a Th1 cytokine predominantly produced by cells in the innate immune system. Its role is linked to the differentiation of naïve T cells into Th1 cells. In allergy, IL-12 is known to downregulate allergic inflammation following its release, along with IFNγ. It modulates the expression of many genes involved in effector T cell function such as FasL and granzyme B. IL-12 signals through the IL-12R activate a STAT4-dependent intracellular pathway, leading to the production of IFNγ but also directly resulting in IL-18 synthesis. It is important for cancer therapy to provide a crucial bridge between innate and adaptive immunity.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.329 2.228 2.279 2.077 1000 1.748 1.647 1.698 1.496 500 1.152 1.131 1.142 0.940 250 0.794 0.783 0.789 0.587 125 0.506 0.495 0.501 0.299 62.5 0.415 0.404 0.410 0.208 31.25 0.308 0.303 0.306 0.104 0 0.202 0.201 0.202

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