产品描述
This Rat INH-B ELISA Kit was designed for the quantitative measurement of Rat INH-B protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1 pg/mL-200 pg/mL and the sensitivity is 0.5 pg/mL.
别名
N/A
缩写
INH-B
灵敏度
0.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-106 |
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| 1:2 |
Average % |
98 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-99 |
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| 1:8 |
Average % |
96 |
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| Range % |
90-100 |
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回收率
| The recovery of rat INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
87-104 |
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| EDTA plasma (n=4) |
97 |
91-106 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.240 |
0.246 |
0.243 |
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| 50 |
0.291 |
0.305 |
0.298 |
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| 16 |
0.421 |
0.432 |
0.427 |
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| 4 |
0.722 |
0.697 |
0.710 |
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| 1 |
1.117 |
1.146 |
1.132 |
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| 0 |
2.188 |
2.164 |
2.176 |
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货期
3-5 working days