产品描述
LGB (磷脂酶B,Lysophosphatidylglycerol Acyltransferase 2) 是一种参与脂肪酸代谢的酶,将磷脂酰甘油酯化为二酰基甘油。LGB作为内质网膜上的磷脂酰转移酶,在细胞脂质代谢、信号转导和细胞凋亡等方面发挥重要作用。在人体内,LGB主要存在于肝脏、肠道和脾脏等组织中。其含量的变化可以反映肝细胞和肠道的脂质代谢水平。
华美生物所提供的Bovine beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB)ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测牛血清、血浆、milk样本中的LGB,其灵敏度为0.129 μg/mL,检测范围为0.156 μg/mL-10 μg/mL。
别名
Beta lactoglobulin B ELISA Kit; Beta-lactoglobulin ELISA Kit; Beta-LG ELISA Kit; BLG ELISA Kit; LACB_BOVIN ELISA Kit; LGB ELISA Kit
缩写
LGB
Uniprot No
P02754
灵敏度
0.129 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine LGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
87-101 |
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| 1:2 |
Average % |
92 |
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| Range % |
90-95 |
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| 1:4 |
Average % |
89 |
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| Range % |
85-93 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-98 |
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回收率
| The recovery of bovine LGB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
93-99 |
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| EDTA plasma (n=4) |
94 |
89-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 10 |
0.173 |
0.168 |
0.171 |
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| 5 |
0.279 |
0.273 |
0.276 |
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| 2.5 |
0.376 |
0.358 |
0.367 |
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| 1.25 |
0.482 |
0.472 |
0.477 |
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| 0.625 |
0.631 |
0.653 |
0.642 |
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| 0.313 |
0.948 |
0.914 |
0.931 |
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| 0.156 |
1.166 |
1.193 |
1.180 |
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| 0 |
1.884 |
1.952 |
1.918 |
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货期
3-5 working days