产品描述

This Human NRP2 ELISA Kit was designed for the quantitative measurement of Human NRP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

MGC126574 ELISA Kit; Neuropilin 2 ELISA Kit; Neuropilin-2 ELISA Kit; Neuropilin2 ELISA Kit; NP 2 ELISA Kit; NP2 ELISA Kit; NPN 2 ELISA Kit; NPN2 ELISA Kit; NRP 2 ELISA Kit; Nrp2 ELISA Kit; NRP2_HUMAN ELISA Kit; PRO2714 ELISA Kit; Receptor for VEGF165 and semaphorins class3 ELISA Kit; Vascular endothelial cell growth factor 165 receptor 2 ELISA Kit; Vascular endothelial growth factor 165 receptor 2 ELISA Kit; VEGF1265R2 ELISA Kit; VEGF165R2 ELISA Kit

缩写

NRP2

Uniprot No

O60462

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NRP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		89-97
1:2		Average %		96
		Range %		91-102
1:4		Average %		92
		Range %		83-96
1:8		Average %		86
		Range %		80-91

回收率

The recovery of human NRP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		98-107
EDTA plasma (n=4)		97		92-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.540 2.432 2.486 2.375 10 1.899 2.044 1.972 1.861 5 1.232 1.276 1.254 1.143 2.5 0.682 0.675 0.679 0.568 1.25 0.386 0.374 0.380 0.269 0.625 0.271 0.282 0.277 0.166 0.312 0.184 0.186 0.185 0.074 0 0.110 0.112 0.111

货期

3-5 working days