产品描述
This Dog NT-ProANP ELISA Kit was designed for the quantitative measurement of Dog NT-ProANP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
缩写
NT-ProANP
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog NT-ProANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 91-105 | |
| 1:2 | Average % | 97 | |
| Range % | 91-100 | |
| 1:4 | Average % | 100 | |
| Range % | 92-106 | |
| 1:8 | Average % | 95 | |
| Range % | 86-98 | |
回收率
| The recovery of dog NT-ProANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-97 | |
| EDTA plasma (n=4) | 99 | 95-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 1.830 | 1.867 | 1.849 | 1.645 | | | 10 | 1.508 | 1.524 | 1.516 | 1.312 | | | 5 | 1.136 | 1.166 | 1.151 | 0.947 | | | 2.5 | 0.829 | 0.812 | 0.821 | 0.617 | | | 1.25 | 0.538 | 0.524 | 0.531 | 0.327 | | | 0.625 | 0.361 | 0.375 | 0.368 | 0.164 | | | 0.312 | 0.263 | 0.278 | 0.271 | 0.067 | | | 0 | 0.205 | 0.203 | 0.204 | | |
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货期
3-5 working days