产品描述

This human programmed death 1 (PD-1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PD-1 in different samples, including serum, plasma, tissue homogenates, or cell lysates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PD-1 bound in the initial step.

PD-1, also called PDCD1, is a type I transmembrane protein preferentially expressed in immune cells such as T, B, and NK cells. PD-1 and its ligand PD-L1 are critical immune checkpoint proteins that mediate inhibition of T cell proliferation, activation, cytokine production, altered metabolism, and cytotoxic T lymphocytes (CTLs) killer functions and eventual death of activated T cells. Tumor cells hijack these inhibitory pathways to escape host immune surveillance through overexpression of PD-L1. Antibodies targeting PD-1 have shown efficacy in increasing the anti-tumor activity of T cells in PD-L1-expressing cancers.

别名

CD279 ELISA Kit; CD279 antigen ELISA Kit; hPD 1 ELISA Kit; hPD l ELISA Kit; hPD-1 ELISA Kit; hSLE1 ELISA Kit; PD 1 ELISA Kit; PD-1 ELISA Kit; PD1 ELISA Kit; PDCD 1 ELISA Kit; PDCD1 ELISA Kit; PDCD1_HUMAN ELISA Kit; Programmed cell death 1 ELISA Kit; Programmed cell death 1 protein ELISA Kit; Programmed cell death protein 1 ELISA Kit; Protein PD 1 ELISA Kit; Protein PD-1 ELISA Kit; SLEB2 ELISA Kit; Systemic lupus erythematosus susceptibility 2 ELISA Kit

缩写

PDCD1

Uniprot No

Q15116

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PD-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		88-98
1:2		Average %		99
		Range %		95-104
1:4		Average %		95
		Range %		87-99
1:8		Average %		90
		Range %		86-94

回收率

The recovery of human PD-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		96-104
EDTA plasma (n=4)		91		87-95

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1000 2.673 2.617 2.645 2.437   500 2.302 2.239 2.271 2.063   250 1.853 1.714 1.784 1.576   125 1.524 1.429 1.477 1.269   62.5 1.057 0.996 1.027 0.819   31.2 0.639 0.682 0.661 0.453   15.6 0.307 0.329 0.318 0.110   0 0.205 0.210 0.208

货期

3-5 working days