产品描述
This Human MMP20 ELISA Kit was designed for the quantitative measurement of Human MMP20 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
AI2A2 ELISA Kit; Enamel metalloproteinase ELISA Kit; Enamelysin ELISA Kit; Matrix metalloproteinase 20 ELISA Kit; Matrix metalloproteinase-20 ELISA Kit; MMP 20 ELISA Kit; MMP-20 ELISA Kit; MMP20 ELISA Kit; MMP20_HUMAN ELISA Kit
缩写
MMP20
Uniprot No
O60882
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 89 | | |
| Range % | 85-93 | | |
| 1:2 | Average % | 100 | | |
| Range % | 95-105 | | |
| 1:4 | Average % | 86 | | |
| Range % | 83-89 | | |
| 1:8 | Average % | 97 | | |
| Range % | 92-101 | | |
回收率
| The recovery of human MMP20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 88 | 86-90 | | |
| EDTA plasma (n=4) | 101 | 97-104 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.303 | 2.204 | 2.254 | 2.101 | | | | 10 | 1.915 | 1.964 | 1.940 | 1.787 | | | | 5 | 1.636 | 1.530 | 1.583 | 1.430 | | | | 2.5 | 1.201 | 1.301 | 1.251 | 1.098 | | | | 1.25 | 0.821 | 0.835 | 0.828 | 0.675 | | | | 0.625 | 0.512 | 0.501 | 0.507 | 0.354 | | | | 0.312 | 0.352 | 0.368 | 0.360 | 0.207 | | | | 0 | 0.154 | 0.152 | 0.153 | | | |
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货期
3-5 working days