产品描述
This Human MG7-Ag ELISA Kit was designed for the quantitative measurement of Human MG7-Ag protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1 U/mL-15 U/mL and the sensitivity is 0.25 U/mL.
缩写
MG7-Ag
灵敏度
0.25 U/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<20% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MG7-Ag in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
83-99 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
87-102 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of human MG7-Ag spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
93 |
88-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| U/ml |
OD1 |
OD2 |
Average |
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| 15 |
2.048 |
2.027 |
2.038 |
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| 10 |
1.392 |
1.388 |
1.390 |
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| 4.5 |
0.728 |
0.731 |
0.730 |
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| 1.5 |
0.304 |
0.307 |
0.306 |
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货期
3-5 working days