产品描述
This Bovine IL4 ELISA Kit was designed for the quantitative measurement of Bovine IL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL .
别名
IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写
IL4
Uniprot No
P30367
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 89-99 | |
| 1:2 | Average % | 97 | |
| Range % | 92-103 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 84 | |
| Range % | 80-88 | |
回收率
| The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 89 | 84-93 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 1.993 | 1.912 | 1.953 | 1.835 | | | 400 | 1.576 | 1.616 | 1.596 | 1.478 | | | 200 | 1.304 | 1.354 | 1.329 | 1.211 | | | 100 | 0.939 | 0.962 | 0.951 | 0.833 | | | 50 | 0.624 | 0.641 | 0.633 | 0.515 | | | 25 | 0.441 | 0.419 | 0.430 | 0.312 | | | 12.5 | 0.283 | 0.279 | 0.281 | 0.163 | | | 0 | 0.119 | 0.117 | 0.118 | | |
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货期
3-5 working days