产品描述
This Mouse IL27 ELISA Kit was designed for the quantitative measurement of Mouse IL27 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
Il27 ELISA Kit; Il27aInterleukin-27 subunit alpha ELISA Kit; IL-27 subunit alpha ELISA Kit; IL-27-A ELISA Kit; IL27-A ELISA Kit; p28 ELISA Kit
缩写
IL27
Uniprot No
Q8K3I6
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 89 | | |
| Range % | 85-93 | | |
| 1:2 | Average % | 106 | | |
| Range % | 103-109 | | |
| 1:4 | Average % | 83 | | |
| Range % | 80-85 | | |
| 1:8 | Average % | 98 | | |
| Range % | 94-102 | | |
回收率
| The recovery of mouse IL-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 100 | 95-105 | | |
| EDTA plasma (n=4) | 94 | 89-98 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 2000 | 2.584 | 2.631 | 2.608 | 2.471 | | | | 1000 | 1.979 | 1.998 | 1.989 | 1.852 | | | | 500 | 1.332 | 1.301 | 1.317 | 1.180 | | | | 250 | 0.782 | 0.774 | 0.778 | 0.641 | | | | 125 | 0.485 | 0.468 | 0.477 | 0.340 | | | | 62.5 | 0.263 | 0.278 | 0.271 | 0.134 | | | | 31.25 | 0.206 | 0.214 | 0.210 | 0.073 | | | | 0 | 0.138 | 0.135 | 0.137 | | | |
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货期
3-5 working days