产品描述
This Rat IL-23 ELISA Kit was designed for the quantitative measurement of Rat IL-23 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
别名
N/A
缩写
IL-23
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 89-94 | |
| 1:2 | Average % | 104 | |
| Range % | 100-107 | |
| 1:4 | Average % | 92 | |
| Range % | 87-95 | |
回收率
| The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-95 | |
| EDTA plasma (n=4) | 98 | 94-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.147 | 2.112 | 2.130 | 1.998 | | | 100 | 1.851 | 1.776 | 1.814 | 1.682 | | | 50 | 1.308 | 1.288 | 1.298 | 1.166 | | | 25 | 0.903 | 0.898 | 0.901 | 0.769 | | | 12.5 | 0.621 | 0.614 | 0.618 | 0.486 | | | 6.25 | 0.375 | 0.359 | 0.367 | 0.235 | | | 3.12 | 0.236 | 0.224 | 0.230 | 0.098 | | | 0 | 0.134 | 0.130 | 0.132 | | |
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货期
3-5 working days