产品描述
This Rabbit IL10 ELISA Kit was designed for the quantitative measurement of Rabbit IL10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写
IL10
Uniprot No
Q9TSJ4
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-98 | |
| 1:2 | Average % | 96 | |
| Range % | 91-105 | |
| 1:4 | Average % | 97 | |
| Range % | 92-110 | |
| 1:8 | Average % | 93 | |
| Range % | 86-100 | |
回收率
| The recovery of rabbit IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 89 | 80-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.627 | 2.549 | 2.588 | 2.461 | | | 1000 | 1.954 | 1.799 | 1.877 | 1.750 | | | 500 | 1.004 | 0.987 | 0.996 | 0.869 | | | 250 | 0.599 | 0.554 | 0.577 | 0.450 | | | 125 | 0.369 | 0.361 | 0.365 | 0.238 | | | 62.5 | 0.274 | 0.264 | 0.269 | 0.142 | | | 31.25 | 0.177 | 0.171 | 0.174 | 0.047 | | | 0 | 0.132 | 0.122 | 0.127 | | |
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货期
3-5 working days