产品描述
This Rabbit IL12/P70 ELISA Kit was designed for the quantitative measurement of Rabbit IL12/P70 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
IL12/P70, Interleukin-12/P70
缩写
IL-12/P70
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 89-95 | |
| 1:2 | Average % | 90 | |
| Range % | 83-99 | |
| 1:4 | Average % | 95 | |
| Range % | 92-102 | |
| 1:8 | Average % | 93 | |
| Range % | 86-99 | |
回收率
| The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 86-110 | |
| EDTA plasma (n=4) | 97 | 89-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.096 | 2.108 | 2.102 | 1.938 | | | 100 | 1.678 | 1.651 | 1.665 | 1.501 | | | 50 | 1.254 | 1.237 | 1.246 | 1.082 | | | 25 | 0.896 | 0.905 | 0.901 | 0.737 | | | 12.5 | 0.614 | 0.642 | 0.628 | 0.464 | | | 6.25 | 0.378 | 0.364 | 0.371 | 0.207 | | | 3.12 | 0.252 | 0.249 | 0.251 | 0.087 | | | 0 | 0.161 | 0.166 | 0.164 | | |
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货期
3-5 working days