产品描述
This Rabbit PAI-1 ELISA Kit was designed for the quantitative measurement of Rabbit PAI-1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .
缩写
PAI-1
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:20 | Average % | 87 | |
| Range % | 83-90 | |
| 1:40 | Average % | 100 | |
| Range % | 97-103 | |
| 1:80 | Average % | 96 | |
| Range % | 90-102 | |
| 1:160 | Average % | 90 | |
| Range % | 85-95 | |
回收率
| The recovery of rabbit PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 80-86 | |
| EDTA plasma (n=4) | 96 | 93-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | | | | 400 | 0.124 | 0.126 | 0.125 | | | | 200 | 0.206 | 0.215 | 0.211 | | | | 100 | 0.368 | 0.350 | 0.359 | | | | 50 | 0.545 | 0.571 | 0.558 | | | | 25 | 0.768 | 0.765 | 0.767 | | | | 12.5 | 0.917 | 0.938 | 0.928 | | | | 6.25 | 1.128 | 1.157 | 1.143 | | | | 0 | 1.586 | 1.599 | 1.593 | | |
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货期
3-5 working days