产品描述
This Human IL19 ELISA Kit was designed for the quantitative measurement of Human IL19 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
IL 10C ELISA Kit; IL 19 ELISA Kit; IL-19 ELISA Kit; Il19 ELISA Kit; IL19_HUMAN ELISA Kit; Interleukin 19 ELISA Kit; Interleukin-19 ELISA Kit; MDA1 ELISA Kit; Melanoma differentiation-associated protein-like protein ELISA Kit; NG.1 ELISA Kit; ZMDA1 ELISA Kit
缩写
IL19
Uniprot No
Q9UHD0
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 89 | | |
| Range % | 84-93 | | |
| 1:2 | Average % | 104 | | |
| Range % | 100-110 | | |
| 1:4 | Average % | 96 | | |
| Range % | 92-100 | | |
| 1:8 | Average % | 90 | | |
| Range % | 85-95 | | |
回收率
| The recovery of human IL-19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 85 | 80-89 | | |
| EDTA plasma (n=4) | 94 | 90-98 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.572 | 2.436 | 2.504 | 2.398 | | | | 500 | 1.831 | 1.725 | 1.778 | 1.672 | | | | 250 | 0.985 | 1.003 | 0.994 | 0.888 | | | | 125 | 0.549 | 0.557 | 0.553 | 0.447 | | | | 62.5 | 0.327 | 0.312 | 0.320 | 0.214 | | | | 31.25 | 0.216 | 0.204 | 0.210 | 0.104 | | | | 15.6 | 0.160 | 0.168 | 0.164 | 0.058 | | | | 0 | 0.103 | 0.109 | 0.106 | | | |
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货期
3-5 working days