产品描述
This Rabbit MMP9 ELISA Kit was designed for the quantitative measurement of Rabbit MMP9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.
别名
MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
缩写
MMP9
Uniprot No
P41246
灵敏度
5.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
99 |
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| Range % |
96-102 |
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| 1:40 |
Average % |
85 |
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| Range % |
92-99 |
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| 1:80 |
Average % |
101 |
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| Range % |
98-104 |
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| 1:160 |
Average % |
85 |
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| Range % |
82-89 |
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回收率
| The recovery of rabbit MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
93-99 |
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| EDTA plasma (n=4) |
100 |
96-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 1500 |
0.129 |
0.127 |
0.128 |
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| 750 |
0.226 |
0.238 |
0.232 |
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| 375 |
0.379 |
0.370 |
0.375 |
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| 187.5 |
0.572 |
0.565 |
0.569 |
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| 94 |
0.799 |
0.785 |
0.792 |
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| 47 |
0.937 |
0.987 |
0.962 |
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| 23.5 |
1.148 |
1.168 |
1.158 |
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| 0 |
1.606 |
1.634 |
1.620 |
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货期
3-5 working days