产品描述

This Human MELK ELISA Kit was designed for the quantitative measurement of Human MELK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

别名

AI327312 ELISA Kit; hMELK ELISA Kit; HPK 38 ELISA Kit; hPK38 ELISA Kit; KIAA0175 ELISA Kit; Likely ortholog of maternal embryonic leucine zipper kinase ELISA Kit; Maternal embryonic leucine zipper kinase ELISA Kit; MELK ELISA Kit; MELK_HUMAN ELISA Kit; mKIAA0175 ELISA Kit; MPK38 ELISA Kit; OTTHUMP00000021377 ELISA Kit; OTTHUMP00000046113 ELISA Kit; pEg3 kinase ELISA Kit; Protein kinase Eg3 ELISA Kit; Protein kinase PK38 ELISA Kit; RP23 382O11.1 ELISA Kit; Tyrosine protein kinase MELK ELISA Kit

缩写

MELK

Uniprot No

Q14680

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MELK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-98
1:2		Average %		86
		Range %		80-93
1:4		Average %		96
		Range %		90-101
1:8		Average %		100
		Range %		96-105

回收率

The recovery of human MELK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		88-99
EDTA plasma (n=4)		95		89-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.776 2.863 2.820 2.671 5 2.121 1.995 2.058 1.909 2.5 1.142 1.241 1.192 1.043 1.25 0.705 0.733 0.719 0.570 0.625 0.393 0.387 0.390 0.241 0.312 0.296 0.284 0.290 0.141 0.156 0.238 0.245 0.242 0.093 0 0.150 0.148 0.149

货期

3-5 working days