产品描述

This mouse LBP ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse LBP in serum, plasma, and tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of LBP bound in the initial step.

LBP is an acute-phase protein mainly produced in hepatocytes that binds to bacterial lipopolysaccharide (LPS) thus initiating an innate host response. LBP combines with LPS and then recruits CD14 and MD-4/2 complex, eventually leading to the activation of signal transduction pathways and the generation of cytokines and other pro-inflammatory mediators. Circulating LBP exerts immunologic function in a concentration-dependent manner. Subnormal concentrations of LBP augment phagocytosis and scavenging of LPS from blood. However, high levels of LBP decrease the release of pro-inflammatory cytokines. LBP is a potential acute-phase biomarker for monitoring the progress of severe sepsis, infectious endocarditis, and cardiovascular disease.

别名

缩写

Uniprot No

灵敏度

反应时间

样本体积

检测波长

研究领域

测定原理

精密度

线性度

回收率

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 800 2.922 2.974 2.948 2.803 400 2.166 2.159 2.163 2.018 200 1.447 1.448 1.448 1.303 100 0.913 0.925 0.919 0.774 50 0.524 0.565 0.545 0.400 25 0.335 0.312 0.324 0.179 12.5 0.253 0.258 0.256 0.111 0 0.140 0.150 0.145

货期