产品描述

This human ING4 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ING4 in multiple samples, including serum, plasma, cell lysates, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the ING4 levels in the sample and is measured at 450 nm through a microplate reader.

ING4 has been identified as a tumor suppressor since it plays a critical role in the regulation of chromatin modification, cell proliferation, angiogenesis, and cell migration. It is frequently down-regulated in various human cancers. Decreased expression or dysregulation of ING4 is widely seen in diverse types of cancers. Ample studies have shown that ING4 is responsible for important cancer hallmarks such as pathologic cell cycle arrest, apoptosis, autophagy, contact inhibition, and hypoxic adaptation, and also affects tumor angiogenesis, invasion, and metastasis.

别名

Brain my036 protein ELISA Kit; Candidate tumor suppressor p33 ING 1 homolog ELISA Kit; Candidate tumor suppressor p33 ING1 homolog ELISA Kit; D6Wsu147e ELISA Kit; D6Xrf92 ELISA Kit; ING 1 like protein ELISA Kit; ING 4 ELISA Kit; ING1 like protein ELISA Kit; ING4 ELISA Kit; ING4_HUMAN ELISA Kit; Inhibitor of growth family member 4 ELISA Kit; Inhibitor of growth family member 4 long isoform ELISA Kit; Inhibitor of growth protein 4 ELISA Kit; MGC12557 ELISA Kit; my036 ELISA Kit; p29 ING 4 ELISA Kit; p29 ING4 ELISA Kit; p29ING4 ELISA Kit

缩写

ING4

Uniprot No

Q9UNL4

灵敏度

11.75 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ING4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		86-92
1:2		Average %		101
		Range %		97-103
1:4		Average %		96
		Range %		92-99
1:8		Average %		91
		Range %		87-95

回收率

The recovery of human ING4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-93
EDTA plasma (n=4)		104		99-108

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 3000 2.714 2.642 2.678 2.565 1500 1.538 1.544 1.541 1.428 750 0.749 0.788 0.769 0.656 375 0.491 0.500 0.496 0.383 187.5 0.301 0.312 0.307 0.194 94 0.224 0.232 0.228 0.115 47 0.183 0.174 0.179 0.066 0 0.114 0.112 0.113

货期

3-5 working days