产品描述
Neurturin, a protein encoded by the NRTN gene in human, plays a critical role in the development and maintenance of dopaminergic neurons and motor neurons. This Human Neurturin ELISA kit provides a reliable and sensitive method for the detection of human Neurturin in a range of sample types, including serum, plasma, and tissue homogenates.
With a detection range of 125 pg/mL to 8000 pg/mL and a sensitivity of 31.25 pg/mL, this ELISA kit enables precise and accurate measurement of Neurturin levels in your samples. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a fast and efficient tool for your research.
The Human Neurturin ELISA kit follows a sandwich assay principle, providing robust and reproducible results. The detection wavelength is 450 nm, ensuring high sensitivity and specificity. This kit is an excellent choice for researchers studying the molecular mechanisms of dopaminergic neurons and other areas of neuroscience.
别名
Neurturin ELISA Kit; Neurturin precursor ELISA Kit; NRTN ELISA Kit; NRTN_HUMAN ELISA Kit; NTN ELISA Kit; prepro-neurturin ELISA Kit
缩写
NRTN
Uniprot No
Q99748
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human NRTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 88-97 | |
| 1:2 | Average % | 103 | |
| Range % | 97-108 | |
| 1:4 | Average % | 97 | |
| Range % | 92-102 | |
| 1:8 | Average % | 94 | |
| Range % | 89-99 | |
回收率
| The recovery of human NRTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 86-97 | |
| EDTA plasma (n=4) | 104 | 98-109 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 8000 | 2.546 | 2.664 | 2.605 | 2.470 | | | 4000 | 2.196 | 2.075 | 2.136 | 2.001 | | | 2000 | 1.464 | 1.489 | 1.477 | 1.342 | | | 1000 | 0.836 | 0.822 | 0.829 | 0.694 | | | 500 | 0.428 | 0.433 | 0.431 | 0.296 | | | 250 | 0.283 | 0.294 | 0.289 | 0.154 | | | 125 | 0.196 | 0.190 | 0.193 | 0.058 | | | 0 | 0.136 | 0.134 | 0.135 | | |
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货期
3-5 working days
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