产品描述
This Rat IL5 ELISA Kit was designed for the quantitative measurement of Rat IL5 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
缩写
IL5
Uniprot No
Q08125
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 82-91 | |
| 1:2 | Average % | 92 | |
| Range % | 83-99 | |
| 1:4 | Average % | 101 | |
| Range % | 93-107 | |
| 1:8 | Average % | 96 | |
| Range % | 90-104 | |
回收率
| The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 88 | 85-94 | |
| EDTA plasma (n=4) | 94 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.407 | 2.457 | 2.432 | 2.268 | | | 500 | 1.768 | 1.736 | 1.752 | 1.588 | | | 250 | 1.065 | 1.098 | 1.082 | 0.918 | | | 125 | 0.704 | 0.735 | 0.720 | 0.556 | | | 62.5 | 0.448 | 0.469 | 0.459 | 0.295 | | | 31.2 | 0.323 | 0.300 | 0.312 | 0.148 | | | 15.6 | 0.221 | 0.229 | 0.225 | 0.061 | | | 0 | 0.161 | 0.167 | 0.164 | | |
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货期
3-5 working days