产品描述
This Pig IL18 ELISA Kit was designed for the quantitative measurement of Pig IL18 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写
IL18
Uniprot No
O19073
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 82-92 | |
| 1:2 | Average % | 100 | |
| Range % | 95-105 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 97 | |
| Range % | 91-103 | |
回收率
| The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 84-93 | |
| EDTA plasma (n=4) | 94 | 91-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.562 | 2.687 | 2.625 | 2.443 | | | 1000 | 1.839 | 1.865 | 1.852 | 1.670 | | | 500 | 1.104 | 1.135 | 1.120 | 0.938 | | | 250 | 0.643 | 0.638 | 0.641 | 0.459 | | | 125 | 0.412 | 0.435 | 0.424 | 0.242 | | | 62.5 | 0.321 | 0.341 | 0.331 | 0.149 | | | 31.25 | 0.262 | 0.248 | 0.255 | 0.073 | | | 0 | 0.181 | 0.183 | 0.182 | | |
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货期
3-5 working days