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收藏| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 107 | |||||
| Range % | 95-111 | ||||||
| 1:40 | Average % | 104 | |||||
| Range % | 95-108 | ||||||
| 1:80 | Average % | 90 | |||||
| Range % | 83-94 | ||||||
| 1:160 | Average % | 97 | |||||
| Range % | 87-103 | ||||||
| The recovery of rat IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 82-94 | |||||
| EDTA plasma (n=4) | 101 | 90-105 | |||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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