产品描述

This Human ITIH2 ELISA Kit was designed for the quantitative measurement of Human ITIH2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 μg/mL-200 μg/mL and the sensitivity is 0.78 μg/mL.

别名

AI747202 ELISA Kit; H2P ELISA Kit; IGHEP2 ELISA Kit; Inter alpha (globulin) inhibitor H2 ELISA Kit; Inter alpha globulin inhibitor H2 polypeptide ELISA Kit; Inter alpha trypsin inhibitor complex component II ELISA Kit; Inter alpha trypsin inhibitor heavy chain H2 ELISA Kit; Inter-alpha-inhibitor heavy chain 2 ELISA Kit; Inter-alpha-trypsin inhibitor complex component II ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H2 ELISA Kit; Intin2 ELISA Kit; ITI HC2 ELISA Kit; ITI heavy chain H2 ELISA Kit; ITI-HC2 ELISA Kit; Itih 2 ELISA Kit; Itih2 ELISA Kit; ITIH2_HUMAN ELISA Kit; OTTHUMP00000019058 ELISA Kit; OTTHUMP00000019059 ELISA Kit; OTTMUSP00000012019 ELISA Kit; RP23 119N4.3 ELISA Kit; Serum derived hyaluronan associated protein ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit

缩写

ITIH2

Uniprot No

P19823

灵敏度

0.78 μg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		91
		Range %		86-94
1:10		Average %		97
		Range %		90-104
1:20		Average %		101
		Range %		96-107
1:40		Average %		89
		Range %		84-94

回收率

The recovery of human ITIH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		88-95
EDTA plasma (n=4)		102		97-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml OD1 OD2 Average Corrected 200 2.439 2.555 2.497 2.312 100 1.867 1.728 1.798 1.613 50 1.221 1.265 1.243 1.058 25 0.751 0.757 0.754 0.569 12.5 0.412 0.422 0.417 0.232 6.25 0.318 0.336 0.327 0.142 3.12 0.272 0.281 0.277 0.092 0 0.183 0.186 0.185

货期

3-5 working days