产品描述
This Human NEFH ELISA Kit was designed for the quantitative measurement of Human NEFH protein in serum, plasma, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
别名
200 kDa neurofilament protein ELISA Kit; CMT2CC ELISA Kit; Nefh ELISA Kit; Neurofilament heavy polypeptide 200kDa ELISA Kit; Neurofilament heavy polypeptide ELISA Kit; Neurofilament triplet H protein ELISA Kit; NF H ELISA Kit; NF-H ELISA Kit; NFH ELISA Kit; NFH_HUMAN ELISA Kit
缩写
NEFH
Uniprot No
P12036
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of Human NF-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 86-98 | |
| 1:2 | Average % | 97 | |
| Range % | 93-101 | |
| 1:4 | Average % | 93 | |
| Range % | 84-97 | |
| 1:8 | Average % | 103 | |
| Range % | 95-107 | |
回收率
| The recovery of Human NF-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 85 | 80-90 | |
| EDTA plasma (n=4) | 94 | 88-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.513 | 2.614 | 2.564 | 2.396 | | | 20 | 2.017 | 2.115 | 2.066 | 1.898 | | | 10 | 1.442 | 1.343 | 1.393 | 1.225 | | | 5 | 0.887 | 0.864 | 0.876 | 0.708 | | | 2.5 | 0.531 | 0.545 | 0.538 | 0.370 | | | 1.25 | 0.374 | 0.362 | 0.368 | 0.200 | | | 0.625 | 0.276 | 0.284 | 0.280 | 0.112 | | | 0 | 0.167 | 0.168 | 0.168 | | |
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货期
3-5 working days