产品描述
This Pig IL1B ELISA Kit was designed for the quantitative measurement of Pig IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 pg/mL-250 pg/mL and the sensitivity is 0.975 pg/mL .
别名
IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
缩写
IL1B
Uniprot No
P26889
灵敏度
0.975 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 80-100 | |
| 1:2 | Average % | 96 | |
| Range % | 91-103 | |
| 1:4 | Average % | 95 | |
| Range % | 90-110 | |
| 1:8 | Average % | 95 | |
| Range % | 85-105 | |
回收率
| The recovery of pig IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 83-104 | |
| EDTA plasma (n=4) | 98 | 90-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 250 | 2.675 | 2.815 | 2.745 | 2.597 | | | 125 | 2.154 | 2.147 | 2.151 | 2.003 | | | 62.5 | 1.312 | 1.335 | 1.324 | 1.176 | | | 31.25 | 0.788 | 0.764 | 0.776 | 0.628 | | | 15.6 | 0.377 | 0.356 | 0.367 | 0.219 | | | 7.8 | 0.264 | 0.269 | 0.267 | 0.119 | | | 3.9 | 0.190 | 0.188 | 0.189 | 0.041 | | | 0 | 0.153 | 0.143 | 0.148 | | |
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货期
3-5 working days