产品描述

This mouse MMP2 ELISA Kit is suitable for qualitatively determining mouse concentrations in multiple biological fluids, including mouse serum, plasma, cell culture supernates, and tissue homogenates in vitro.IL27 is a heterodimeric cytokine that plays a role both in innate and adaptive immunity. MMP2 is an enzyme that degrades bone matrix, promotes osteoclastogenesis, and enhances osteolysis in bone metastasis by activating multiple signaling pathways. It plays an essential role in extracellular matrix remodeling and other pathological processes, such as tumor progression and skeletal dysplasia. MMP2 is important for cancer progression, invasion, and metastasis. Excessive activation of MMP2 facilitates osteolytic metastasis and bone destruction in late-stage cancers, while its loss-of-function mutations lead to the declined bone mineralization and generalized osteolysis occurring progressively in skeletal developmental disorders, especially in multicentric osteolysis, nodulosis, and arthropathy (MONA). Either upregulation or downregulation of MMP2 activity can cause the same osteolytic effects.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of mouse MMP2 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-mouse MMP2 antibody. Biotin-labeled MMP2 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the MMP2 in the mouse samples. MMP2 binds to the biotinylated anti-MMP2 mouse monoclonal antibody. And the biotin on the biotinylated anti-MMP2 mouse monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of MMP2 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

别名

缩写

Uniprot No

灵敏度

反应时间

样本体积

检测波长

研究领域

测定原理

精密度

线性度

回收率

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.754 2.708 2.731 2.608 500 1.843 1.911 1.877 1.754 250 1.118 1.074 1.096 0.973 125 0.679 0.652 0.666 0.543 62.5 0.375 0.357 0.366 0.243 31.2 0.292 0.308 0.300 0.177 15.6 0.195 0.212 0.204 0.081 0 0.125 0.121 0.123

货期