产品描述

This human ANP ELISA Kit is suitable for qualitatively determining human concentrations in serum and cell culture supernates in vitro. ANP, also called NPPA, is synthesized mainly in the cardiac atria and is released into circulation in response to volume expansion and elevated atrial distention. It mainly functions to cause a decline in expanded extracellular fluid (ECF) volume by increasing renal sodium excretion. Potent natriuretic, diuretic, vasodilator, sympatholytic, and renin- and aldosterone-suppressing activities of ANP tend to lower blood pressure. Studies conducted on animal models have shown that ANP has an important role in regulating volume and electrolyte balance, as well as blood pressure in normotensive subjeers. ANP also modulates the permeability of the systemic vasculature, cellular growth, cellular proliferation, and cardiac hypertrophy.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human ANP in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human ANP antibody. Biotin-labeled ANP antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the ANP in the human samples. ANP binds to the biotinylated anti-ANP human monoclonal antibody. And the biotin on the biotinylated anti-ANP human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of ANP in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

别名

ANF ELISA Kit; ANF_HUMAN ELISA Kit; ANP ELISA Kit; ATFB6 ELISA Kit; Atrial natriuretic factor ELISA Kit; Atrial natriuretic peptide ELISA Kit; Atriopeptin ELISA Kit; Cardiodilatin ELISA Kit; Cardiodilatin related peptide ELISA Kit; Cardiodilatin-related peptide ELISA Kit; Cardionatrin ELISA Kit; CDD ANF ELISA Kit; CDD ELISA Kit; CDD-ANF ELISA Kit; CDP ELISA Kit; Natriuretic peptide A ELISA Kit; Natriuretic peptide precursor A ELISA Kit; NPPA ELISA Kit; PND ELISA Kit; Prepronatriodilatin ELISA Kit

缩写

NPPA

Uniprot No

P01160

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		80-92
1:2		Average %		97
		Range %		91-105
1:4		Average %		89
		Range %		85-98
1:8		Average %		101
		Range %		95-108

回收率

The recovery of human ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		87-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.932 2.789 2.861 2.771 1000 2.178 2.132 2.155 2.065 500 1.566 1.555 1.561 1.471 250 0.789 0.778 0.784 0.694 125 0.462 0.456 0.459 0.369 62.5 0.278 0.267 0.273 0.183 31.25 0.226 0.218 0.222 0.132 0 0.093 0.087 0.090

货期

3-5 working days