产品描述
This Rat IL15 ELISA Kit was designed for the quantitative measurement of Rat IL15 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .
别名
Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
缩写
IL15
Uniprot No
P97604
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 90-101 | |
| 1:2 | Average % | 93 | |
| Range % | 87-99 | |
| 1:4 | Average % | 87 | |
| Range % | 82-94 | |
| 1:8 | Average % | 100 | |
| Range % | 95-104 | |
回收率
| The recovery of rat IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 91-101 | |
| EDTA plasma (n=4) | 93 | 87-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.511 | 2.567 | 2.539 | 2.383 | | | 50 | 1.773 | 1.783 | 1.778 | 1.622 | | | 25 | 1.106 | 1.154 | 1.130 | 0.974 | | | 12.5 | 0.704 | 0.725 | 0.715 | 0.559 | | | 6.25 | 0.415 | 0.431 | 0.423 | 0.267 | | | 3.12 | 0.287 | 0.297 | 0.292 | 0.136 | | | 1.56 | 0.214 | 0.231 | 0.223 | 0.067 | | | 0 | 0.155 | 0.157 | 0.156 | | |
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货期
3-5 working days