产品描述

This Human NQO1 ELISA Kit was designed for the quantitative measurement of Human NQO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.

别名

Azoreductase ELISA Kit; Cytochrome b 5 reductase ELISA Kit; DHQU ELISA Kit; DIA 4 ELISA Kit; DIA4 ELISA Kit; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) ELISA Kit; Diaphorase (NADH/NADPH) ELISA Kit; Diaphorase 4 ELISA Kit; Dioxin inducible 1 ELISA Kit; DT diaphorase ELISA Kit; DT-diaphorase ELISA Kit; DTD ELISA Kit; Menadione reductase ELISA Kit; NAD(P)H dehydrogenase [quinone] 1 ELISA Kit; NAD(P)H dehydrogenase quinone 1 ELISA Kit; NAD(P)H menadione oxidoreductase 1 dioxin inducible ELISA Kit; NAD(P)H quinone dehydrogenase 1 ELISA Kit; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 ELISA Kit; NAD(P)H:menadione oxidoreductase 1 ELISA Kit; NAD(P)H:Quinone acceptor oxidoreductase type 1 ELISA Kit; NAD(P)H:quinone oxidoreductase 1 ELISA Kit; NAD(P)H:quinone oxireductase ELISA Kit; NMOR 1 ELISA Kit; NMOR I ELISA Kit; NMOR1 ELISA Kit; NMORI ELISA Kit; NQO 1 ELISA Kit; NQO1 ELISA Kit; NQO1_HUMAN ELISA Kit; Phylloquinone reductase ELISA Kit; QR 1 ELISA Kit; QR1 ELISA Kit; Quinone reductase 1 ELISA Kit

缩写

NQO1

Uniprot No

P15559

灵敏度

9.75 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NQO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		89-101
1:2		Average %		89
		Range %		91-94
1:4		Average %		90
		Range %		82-94
1:8		Average %		94
		Range %		84-99

回收率

The recovery of human NQO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		84-95
EDTA plasma (n=4)		99		91-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2500 2.633 2.551 2.592 2.450 1250 2.093 1.976 2.035 1.893 625 1.398 1.345 1.372 1.230 312 0.733 0.747 0.740 0.598 156 0.456 0.494 0.475 0.333 78 0.278 0.292 0.285 0.143 39 0.221 0.217 0.219 0.077 0 0.144 0.140 0.142

货期

3-5 working days