产品描述
This Mouse MMP10 ELISA Kit was designed for the quantitative measurement of Mouse MMP10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transin-2 ELISA Kit
缩写
MMP10
Uniprot No
O55123
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 105 | | |
| Range % | 101-114 | | |
| 1:2 | Average % | 91 | | |
| Range % | 86-95 | | |
| 1:4 | Average % | 98 | | |
| Range % | 94-102 | | |
| 1:8 | Average % | 87 | | |
| Range % | 82-93 | | |
回收率
| The recovery of mouse MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 88-96 | | |
| EDTA plasma (n=4) | 84 | 80-88 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.517 | 2.401 | 2.459 | 2.246 | | | | 500 | 1.791 | 1.736 | 1.764 | 1.551 | | | | 250 | 1.105 | 1.058 | 1.082 | 0.869 | | | | 125 | 0.683 | 0.662 | 0.673 | 0.460 | | | | 62.5 | 0.412 | 0.409 | 0.411 | 0.198 | | | | 31.25 | 0.329 | 0.340 | 0.335 | 0.122 | | | | 15.6 | 0.268 | 0.273 | 0.271 | 0.058 | | | | 0 | 0.210 | 0.215 | 0.213 | | | |
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货期
3-5 working days