产品描述
IL10(Interleukin-10,白细胞介素10)是一种由多种免疫细胞产生的细胞因子,具有抗炎和免疫调节的作用。IL-10主要由T细胞、B细胞、单核细胞和巨噬细胞等细胞合成,并对免疫细胞和炎症反应起调节作用。IL-10能够抑制炎症细胞产生炎症介质,减轻炎症反应,对免疫系统的平衡和调节具有重要作用。IL-10还参与了一系列免疫相关疾病的发生和发展,如自身免疫病、炎症性肠病和过敏性疾病等。
华美生物所提供的Chicken Interleukin 10,IL-10 ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测鸡血清、血浆、组织匀浆样本中的IL10,其灵敏度为0.5 pg/mL,检测范围为1 pg/mL-200 pg/mL。
别名
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit
缩写
IL10
Uniprot No
Q6A2H4
灵敏度
0.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:2 |
Average % |
97 |
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| Range % |
94-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
92 |
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| Range % |
87-96 |
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回收率
| The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
97 |
93-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.155 |
0.152 |
0.154 |
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| 50 |
0.334 |
0.348 |
0.341 |
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| 16 |
0.515 |
0.502 |
0.509 |
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| 4 |
0.969 |
0.975 |
0.972 |
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| 1 |
1.436 |
1.463 |
1.450 |
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| 0 |
2.658 |
2.667 |
2.663 |
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货期
3-5 working days