产品描述
This Rat NT-proBNP ELISA Kit was designed for the quantitative measurement of Rat NT-proBNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
缩写
NT-proBNP
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 84-95 | |
| 1:2 | Average % | 96 | |
| Range % | 90-101 | |
| 1:4 | Average % | 99 | |
| Range % | 94-105 | |
| 1:8 | Average % | 84 | |
| Range % | 80-88 | |
回收率
| The recovery of rat NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-94 | |
| EDTA plasma (n=4) | 94 | 89-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.769 | 2.651 | 2.710 | 2.548 | | | 250 | 1.973 | 1.901 | 1.937 | 1.775 | | | 125 | 1.183 | 1.217 | 1.200 | 1.038 | | | 62.5 | 0.793 | 0.841 | 0.817 | 0.655 | | | 31.2 | 0.506 | 0.550 | 0.528 | 0.366 | | | 15.6 | 0.404 | 0.379 | 0.392 | 0.230 | | | 7.8 | 0.261 | 0.245 | 0.253 | 0.091 | | | 0 | 0.165 | 0.159 | 0.162 | | |
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货期
3-5 working days