产品描述
This Rat IL21 ELISA Kit was designed for the quantitative measurement of Rat IL21 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Il21Interleukin-21 ELISA Kit; IL-21 ELISA Kit
缩写
IL21
Uniprot No
A3QPB9
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 85-96 | |
| 1:2 | Average % | 89 | |
| Range % | 84-95 | |
| 1:4 | Average % | 103 | |
| Range % | 99-108 | |
| 1:8 | Average % | 99 | |
| Range % | 94-105 | |
回收率
| The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 92-101 | |
| EDTA plasma (n=4) | 92 | 88-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.601 | 2.544 | 2.573 | 2.456 | | | 500 | 1.921 | 1.952 | 1.937 | 1.820 | | | 250 | 1.198 | 1.122 | 1.160 | 1.043 | | | 125 | 0.735 | 0.709 | 0.722 | 0.605 | | | 62.5 | 0.425 | 0.459 | 0.442 | 0.325 | | | 31.2 | 0.272 | 0.257 | 0.265 | 0.148 | | | 15.6 | 0.181 | 0.172 | 0.177 | 0.060 | | | 0 | 0.116 | 0.118 | 0.117 | | |
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货期
3-5 working days